Fungal mycotoxins, common food contaminants, contribute to food losses. Particular the aflatoxins (AF) B1, B2, G1 and G2 synthesized by Aspergillus sp. have an adverse effect on human and animal health, including the development of hepatotoxicity and cancer. Cases have been reported in which a single administration of AF resulted in changes in clinical outcomes, serum biochemistry, histology, and hematology. In our experiment, aflatoxicosis was studied in 24 4-week-old Polish Landrace piglets from 6 litters, randomly assigned to 3 experimental groups (N = 6) receiving: 0, 30 and 60 µg AFB1/kg b.w. for 28 consecutive days. The animals were slaughtered, weighed and samples taken for analysis. AFB1 administration had no effect on feed intake, daily gain and red blood cell indices (Hb, RBC, MCV, MCV, Ret) in all groups. Statistically significant differences were observed in the WBC count in the group receiving 60 µg AFB1/kg b.w. compared to the other groups. The analysis of the content of non-heme and heme iron in tissues and plasma iron did not change, although an increasing trend was observed in the group of piglets receiving the highest dose of AFB1. Similarly, the highest dose resulted in nonsignificant but higher levels of nonheme iron in duodenal scrapings. Preliminary conclusions indicate that low concentrations of AFB1 do not disrupt iron homeostasis, although this dose confirms a complex, direct toxic effect causing neutrophil mobilization. Promising studies are currently underway with an actual feed/food contaminating dose of 120 µg AFB1/kg bw.